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Quantitative real-time RT-PCR for genes of interest. Shown are the relative n-fold changes in gene expression in the clinical isolates (S2 versus S1) and in two pairs of transformants expressing the UPC2S2-1 or UPC2S1-1 alleles in the SC5314 background (UPC2M2K31A versus UPC2M2K21A and UPC2M2K31B versus UPC2M2K21B). Error bars represent the standard error of the mean. Asterisks denote statistical significance by Student's t test (P ≤ 0.05). vs., versus.
