TABLE 3.
Expression assays of putative pheromone precursors (ppg1 and ppg2) and receptors (pre1 and pre2) in different cell types of different ages on carrot agar
| Sample | ppg1a | ppg2 | pre1 | pre2 |
|---|---|---|---|---|
| 3 DAIb (without inductionc) | ||||
| Mycelia | −d | − | − | − |
| Ungerminated conidia | − | − | − | + |
| 6 DAI (3 days after induction) | ||||
| Mycelia | − | − | − | ++ |
| Ungerminated conidia | − | − | − | ++ |
| Germinating conidia | ++++ | − | − | ++ |
| Young perithecia | − | − | − | +++ |
| 13 DAI (10 days after induction) | ||||
| Mycelia | − | − | − | ++ |
| Ungerminated conidia | − | − | − | ++ |
| Germinating conidia | ++++ | − | − | ++ |
| Mature perithecia | − | − | − | ++ |
| Ascospores in perithecia | − | − | − | + |
| Ascospores discharged from perithecia | +++ | − | + | + |
| Old culture without induction (15 DAI) | ||||
| Mycelia | + | − | − | ++ |
| Ungerminated conidia | + | − | − | ++ |
| Germinating conidia | ++++ | − | − | ++ |
| Mature perithecia | − | − | − | ++ |
| Ascospores in perithecia | − | − | − | + |
| Ascospores discharged from perithecia | +++ | − | + | + |
a
ppg1, ppg2, pre1, and pre2 expression was assayed with GFP reporter constructs Δppg1GFPR, Δppg2GFPR, Δpre1GFPR, and Δpre2GFPR, respectively.
b
DAI, days after inoculation.
c
Induction by knockdown of mycelia with 500 μl of 2.5% Tween 60 solution to induce sexual development.
d
−, GFP fluorescence signal not detected; +, weak fluorescence; ++, moderate fluorescence; +++, strong fluorescence; ++++, very strong fluorescence; NT, not tested.