FIG. 2.

EMSA of promoter regions containing putative Rim101 binding sites. Protein extracts from the wild-type (WT) (DAY286; lanes 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, and 17) or rim101Δ/rim101Δ (DAY5; lanes 3, 6, 9, 12, 15, and 18) strain grown at pH 8 were incubated with radiolabeled DNA probes for endogenous (+) (lanes 1, 3, 4, 6, 7, and 9) or mutated (−) (lanes 2, 5, and 8) FRE2 (−876, −443, −22) oligomers or endogenous (+) (lanes 10, 12, 13, 15, 16, and 18) or mutated (−) (lanes 11, 14, and 17) FRP1 (−981, −626, −164) oligomers and analyzed by EMSA.