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Deletion analysis of the FRP1 promoter. β-Galactosidase assays were performed with the PFRP1-lacZ and derivative reporter strains grown at pH 4 or pH 8 or at pH 4 with 200 μM BIP. At least three independent transformants were used to determine average Miller units and standard deviation. n-fold induction was determined by comparison of the expression a given construct in either pH-8- or BIP-grown cells to expression in pH-4-grown cells.
