FIG. 5.

EMSAs of the region predicted to contain the BIP-dependent site. Thirty-base-pair oligomers spanning −220/−190, −200/−170, −180/−150, −160/−130, −140/−110, and −120/−90 in relation to the FRP1 START codon were incubated with protein extracts from wild-type C. albicans (DAY286) grown at pH 4 (lanes 1, 4, 7, 10, 13, and 16), pH 4 with 200 μM BIP (lanes 2, 5, 8, 11, 14, and 17), or pH 8 (lanes 3, 6, 9, 12, 15, and 18). Free probe (open arrowhead), a nonspecific gel shift (closed arrowhead), and specific gel shifts (arrows A to C) are indicated.