FIG. 6.

Competition assays between the −220/−190 and −160/−130 regions. Radiolabeled probes for the −160/−130 region (lanes 1 to 7) and −220/−190 region (lanes 8 to 14) were incubated with protein extracts from wild-type (DAY286) cells and −160/−130 region (lanes 2 to 4 and 12 to 14) or −220/−190 region (lanes 5 to 7 and 9 to 11) cold competitor. Competitor was added at 50-fold (lanes 2, 5, 9, and 12), 100-fold (lanes 3, 6, 10, and 13), or 500-fold (lanes 4, 7, 11, and 14) excess. Control reactions lacking competitor are included (lanes 1 and 8).