FIG. 9.

Hap43 is required for iron limitation-specific promoter binding. EMSAs of the −160/−130 probe incubated with protein extracts derived from wild-type (WT) (DAY286; lanes 1 to 3), hap5Δ/hap5Δ (DAY1062; lanes 4 to 6), hap41Δ/hap41Δ (DAY1083; lanes 7 to 9), or hap43Δ/hap43Δ (DAY1085; lanes 10 to 12) cells are shown. Protein extracts were obtained from cells grown in M199 (pH 4) (lanes 1, 4, 7, and 10), M199 (pH 4) plus 200 μM BIP (lanes 2, 5, 8, and 11), or M199 (pH 8) medium (lanes 3, 6, 9, and 12).