TABLE 2.
Sequences of primers used in this study
| Primer | Sequencea |
|---|---|
| FRE2−22 5a | tgtacaattttcaacaCCAAGaagagtttcctaaa |
| FRE2−22 3a | tttaggaaactcttCTTGGtgttgaaaattgtaca |
| FRE2−22 mut5a | tgtacaattttcaacaGCCGGCagagtttcctaaa |
| FRE2−22 mut3a | tttaggaaactctGCCGGCtgttgaaaattgtaca |
| FRE2−443 5a | tttttataaattaatGCCAAGccatttttatttct |
| FRE2−443 3a | agaaataaaaatggCTTGGCattaatttataaaaa |
| FRE2−443 mut5a | tttttataaattaatGCCGGCccatttttatttct |
| FRE2−443 mut3a | agaaataaaaatggGCCGGCattaatttataaaaa |
| FRE2−876 5a | agaactaattgaaaGCCAAGaaaagttatatggaa |
| FRE2−876 3a | ttccatataacttttCTTGGCtttcaattagttct |
| FRE2−876 mut5a | agaactaattgaaaGCCGGCaaaagttatatggaa |
| FRE2−876 mut3a | ttccatataacttttGCCGGCtttcaattagttct |
| PFRP1−981 5a | ggtacatcatgaatCCAAGaattaccgatccgatt |
| PFRP1−981 3a | aatcggatcggtaattCTTGGattcatgatgtacc |
| PFRP1−981 mut 5a | ggtacatcatgaatGCCGGCattaccgatccgatt |
| PFRP1−981 mut 3a | aatcggatcggtaatGCCGGCttcatgatgtacc |
| PFRP1−636 5a | aacacaacgaagtaGCCAAGaatctggcactagca |
| PFRP1−636 3a | tgctagtgccagattCTTGGCtacttcgttgtgtt |
| PFRP1−636 mut 5a | aacacaacgaagtaGCCGGCaatctggcactagca |
| PFRP1−636 mut 3a | tgctagtgccagattGCCGGCtacttcgttgtgtt |
| PFRP1−164 5a | cgcagttttcggattCTTGGCatagaaacgataaa |
| PFRP1−164 3a | atttatcgtttctatGCCAAGaatccgaaaactgcg |
| PFRP1−164 mut 5a | cgcagttttcggattGCCGGCatagaaacgataaa |
| PFRP1−164 mut 3a | tttatcgtttctatGCCGGCaatccgaaaactgcg |
| PFRE2−876 m1 | ataatagaactaattgaaaGCCGGCaaaagttatatggaattgct |
| PFRE2−876 m2 | agcaattccatataacttttGCCGGCtttcaattagttctattat |
| PFRE2−443 m1 | tcctttttttataaattaatGCCGGCccatttttatttcttacag |
| PFRE2−443 m2 | ctgtaagaaataaaaatggGCCGGCattaatttataaaaaaagga |
| PFRP1−981 m1 | cgggaattcgaatGCCGGCattaccgatccgattcctcct |
| PFRP1−981 m2 | aggaggaatcggatcggtaatGCCGGCttcatgatgtacc |
| PFRP1−636 m1 | aactgaacacaacgaagtaGCCGGCaatctggcactagcaacatc |
| PFRP1−636 m2 | gatgttgctagtgccagattGCCGGCtacttcgttgtgttcagtt |
| PFRP1−164 m1 | aatttcgcagttttcggattGCCGGCatagaaacgataaatccac |
| PFRP1−164 m2 | gtggatttatcgtttctatGCCGGCaatccgaaaactgcgaaatt |
| FRP1-1 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgatttgatgagcctgtagatgacg |
| FRP1-2 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattgcacttcctggagcagtaac |
| FRP1-3 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattaagcaggcatcatccaagta |
| FRP1-4 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattacattgaccaactaaaccag |
| FRP1-5 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgatttttgcacagtctggtaataa |
| FRP1-6 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattcagcagatactaaaagaatc |
| FRP1-7 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattgttaaacccgaaagaaaagt |
| FRP1-8 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattgtaaaatttcgcagttttcg |
| FRP1-9 | ggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgatttgcatgaatgaaaagtatta |
| LacZClaI+200c | tacggaaataatcagccatgtcagc |
| FRP1-8-1-5a′ | taaaaatgcttttgtgctgcgtaaaatttc |
| FRP1-8-1-3a′ | gaaattttacgcagcacaaaagcattttta |
| FRP1-8-2-5a′ | gtaaaatttcgcagttttcggattCTTGGC |
| FRP1-8-2-3a′ | GCCAAGaatccgaaaactgcgaaattttac |
| FRP1-8-3-5a′ | gattCTTGGCatagaaacgataaatccacc |
| FRP1-8-3-3a′ | ggtggatttatcgtttctatGCCAAGaatc |
| FRP1-8-4-5a′ | taaatccaccaattaaacgatggccgcatc |
| FRP1-8-4-3a′ | gatgcggccatcgtttaattggtggattta |
| FRP1-8-5-5a′ | tggccgcatcccttgtctgatgataataag |
| FRP1-8-5-3a′ | cttattatcatcagacaagggatgcggcca |
| FRP1-8-6-5a′ | tgataataaggtttattgaatgcatgaatg |
| FRP1-8-6-3a′ | cattcatgcattcaataaaccttattatca |
| FRP1-8-4-mut5a′ | taaatccaTGCGCtaaacgatggccgcatc |
| FRP1-8-4-mut3a′ | gatgcggccatcgtttaGCGCAtggattta |
| HAP5 5DR | aacaaacattcaattaagacgaagaaatacagaaagaccccccaaaacaatactcaaacatttcccagtcacgacgtt |
| HAP5 3DR | tattattacaaaatcaaacactatttttaaaatgaacgaaaaaaaaaaaaaaatcctctagtggaattgtgagcggata |
| HAP5 5detect | aaaatgcgacaacggaaagg |
| HAP5 3detect | caagtgaggaactgagaactg |
| 5′SnaBI CaHIS1 | gtagtggaagatattctttattgaaaaatagcttgtcaccggatcctggaggatgaggag |
| 3-detect | tgtggaattgtgagcggataacaatttcac |
| HAP41 5DR | tacagtatatttattttttcccccatacattaattattctaatcttatgaaattaccatttttcccagtcacgacgtt |
| HAP41 3DR | catctcatcacatcacatcacatcaaaaataaaatccgttaattttttactgttccttcagtggaattgtgagcggata |
| HAP43 5DR | caaaaaaaaacaacagagtccaaaaaaatacataataattagaatttcaatttgaacaactttcccagtcacgacgtt |
| HAP43 3DR | gaaaagaaaagaaaaaaaaaactgaagtgtcggaaatacttcatactgtaagtcaaactagtggaattgtgagcggata |
| HAP41 5detect new | ccactagttcactcaattac |
| HAP41 3detect new | ccaaatcgagcaaaatcaaacc |
| HAP43 5detect | tgtcttccccccaatagact |
| HAP43 3detect | tacctttttgaatacattgt |
| HAP41UPFWD2ND | tgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgactgaaatttctataattttagcagaa |
| HAP41UPREV2ND | ttttaaagaaagtagtaccagctcttttttttgtttccgtttataccatccaaatcccgcaatggtaatttcataagattagaat |
| HAP41DOWNFWD | aataattaaataagggtggtaattattactatttacaatcaaaggtggtccttctagatgtgaaggaacagtaaaaaattaacgg |
| HAP41DOWNREV | ttacactttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacgtgcgtagtgttcggaagtgttgag |
| HAP43UPFWD | tgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacgctgacacaaacgggaacagaaata |
| HAP43UPREV2ND | ttttaaagaaagtagtaccagctcttttttttgtttccgtttataccatccaaatcccgcgttgttcaaattgaaattctaatta |
| HAP43DOWNFWD | aataattaaataagggtggtaattattactatttacaatcaaaggtggtccttctagatgtagtttgacttacagtatgaagtat |
| HAP43DOWNREV | ttacactttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcactaaaggtattggtgctgccgcttat |
| URA3FWD2ND | gcgggatttggatggtataaacgg |
| URA3REV | catctagaaggaccacctttgattg |
a
Uppercase letters denote the endogenous or mutated putative Rim101 binding site.