FIG. 6.

Averaged images of outer doublet cross sections. (A) The statistical difference between outer-arm dyneins from oda2-t and the wild type. Two typical wild-type images (first column) and two typical images of oda2-t (second column) obtained by cluster analysis are shown. The red contours on the wild-type images (third column) show the differences between the wild type and oda2-t at a 99% confidence level determined by the Student t test. The difference in density between the wild type and oda2-t (fourth column) are superimposed onto the outlines of the wild-type images. The motor domain of the γ DHC is most likely located in the proximal region of the outer arm indicated by the green arrows. (B) Averaged outer-arm images from the wild-type (wt), oda11, oda4-s7, and oda2-t axonemes. The averaged images of the largest cluster are shown. The number of images used was 22 (wild type), 23 (oda11), 17 (oda4-s7), and 22 (oda2-t). Bar, 10 nm.