FIG. 2.

Differential expression of SL metabolic genes in nonencysting, preencysting, and encysting trophozoites: expression of gSPT-1 (gspt-1), gSPT-2 (gspt-2), gGlct-1 (gglct-1), gSmase B (gsmase B), and gSmase 3b (gsmase 3b) transcripts in preencysting and encysting trophozoites of G. lamblia relative to the expression in nonencysting (vegetative) trophozoites. The data are the means and standard deviations of three separate experiments, and each experiment was carried out in triplicate. The qRT-PCR analysis of SL genes was carried out as described in Materials and Methods. An asterisk indicates that there were significant differences compared with vegetative trophozoites (P < 0.05).