FIG. 6.

PPMP and PDMP alter gglct-1 and CWP (cwp) transcripts. Giardia trophozoites (∼1 × 10⁷ cells) were cultured and subjected to preencystation for 48 h and then encystation for 12 h using the protocol of Gillin et al. (15) in the presence and absence of inhibitors (10 μM). RNA was isolated, reverse transcribed, and subjected to qRT-PCR analyses as described in Materials and Methods. The data are the means and standard deviations of three individual experiments, and each experiment was carried out in triplicate. An asterisk indicates that there were significant differences compared with vegetative trophozoites (P < 0.05).