TABLE 1.
Molecular beacon and primer sequences
| Oligonucleotide | Sequence (5′-3′)a |
|---|---|
| pks1-15-F | GCATCTGATCTTGGAAGAGG |
| pks1-15-R | AACGCCTCAGCCGATCTC |
| pks1-15c-MB | FAM-CGCGAGCGGCCGTCGATGGTGCCGTGTCGCG-Dabcyl |
| pks1-15-MB | HEX-CGAGCAGCCGCGGGCCGCGGCCGTCTGCTCG-Dabcyl |
| sigA-F | TGCAGTCGGTGCTGGACAC |
| sigA-R | CGCGCAGGACCTGTGAGCGG |
| sigA-MB | HEX-CCTCGCGTCGAAGTTGCGCCATCCGAGCGAGG-Dabcyl |
a
Underlined sequences represent stem structure of the molecular beacons. FAM, fluorescein; HEX, hexachlorofluorescein; Dabcyl, 4-([4-{dimethylamino}phenyl]azo)-benzoyl.