FIG. 2.

EBH induction of cif gene and Cif protein expression. Wild-type (WT) P. aeruginosa strain PA14 was grown in LB supplemented with increasing concentrations of the epoxide EBH. Expression of the cif gene and Cif protein in response to EBH was assayed using either sqRT-PCR (A) or Western blotting (B), respectively. The expression of rplU, a gene previously shown to be constitutively expressed under most laboratory conditions, served as a control (panel A). Samples for Western blotting were normalized to total protein content (panel B). The graphical interpretation of the Western blot data shown in panel B is the relative Cif expression normalized to the total protein electrophoresed.