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Clinical isolates overexpressing the cif gene demonstrate decreased cifR gene expression. (A) sqRT-PCR of wild-type P. aeruginosa PA14 and four nonmucoid P. aeruginosa CF sputum isolates. Expression of the cif, cifR, and rplU genes was assayed. (B) sqRT-PCR of wild-type (WT) P. aeruginosa, the Δcif and ΔcifR mutants, and two of the clinical isolates from panel A carrying either the empty vector pMQ71 (−) or the cifR-expressing plasmid pDPM79 (+).
