TABLE 1.

Strains, plasmids, and primers used

Strain, plasmid, or primer	Relevant characteristic	Reference or source
Strain
S. cerevisiae INVSc1	MATahis3D1 leu2 trp1-289 ura3-52 MAT his3D1 leu2 trp1-289 ura3-52	Invitrogen
E. coli Top10	F−mcrA Δ(mrr-hsdRMS-mcrBC) f80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara leu)7697 galU galK rpsL (StrR) endA1 nupG	Invitrogen
SMC3505	E. coli Top10 + pDPM73 (Cif-His)	29
SMC3814	E. coli Top10 + pDPM79 (CifR-His)	This study
P. aeruginosa PA14	Wild type
SMC1584	P. aeruginosa clinical isolate	29
SMC1586	P. aeruginosa clinical isolate	29
SMC1591	P. aeruginosa clinical isolate	29
SMC1593	P. aeruginosa clinical isolate	29
SMC3816	PA14 ΔPA2931 + pDPM79	This study
SMC3817	SMC1584 + pMQ71	This study
SMC3818	SMC1584 + pDPM79	This study
SMC3819	SMC1586 + pMQ71	This study
SMC3820	SMC1586 + pDPM79	This study
Plasmid
pMQ71	Arabinose-inducible expression vector Cbr Apr URA3	40
pMQ30	Allelic replacement vector; Gmr	40
pDPM79	PA2931 hexa-histidine fusion in pMQ71; Apr Cbr URA3	This study
pDPM84	PA2931 in-frame deletion plasmid; Gmr	This study
Primer
PA2934_rt_1	5′-CTCCTGGCCGGCATCGCCCTGACCTTCTCC-3′
PA2934_rt_2	5′-CCATTCGTACCAGGTCTGGCCGAAGCCGTGC-3′
RplU_rt_1	5′-GGTGGCAAGCAGCACAAAGTCACCG-3′
RplU_rt_2	5′-GCGGACCTTGTCGTGACGGCCGTGG-3′
PA2931_rt_for	5′-CCAGGGCTTCGCGGAACAGCCCTTCC-3′
PA2931_rt_rev	5′-GACACCGCCCTGCAGCGAGCCATGG-3′
Cif_northern_for	5′-BT-GCCGCGGCCTCCTGGCCGGCATCGCCC-3′a
Cif_northern_rev	5′-BT-CGGCGACCGTTCGCCGCTATAGCCGG-3′
PA2931_ko_1	5′-CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACG GCTGGAAGGCTCCAAGGTAGAATGGATGG-3′
PA2931_ko_2	5′-CAAGTAAATTTATAGTGATCGATACAAATATGAGCG GGTCGCATCAGGCCGCC-3′
PA2931_ko_3	5′-GGCGGCCTGATGCGACCCGCTCATATTTGTATC GATCACTATAAATTTACTTG-3′
PA2931_ko_4	5′-GTGAGCGGATAACAATTTCACACAGGAAACAGCTAT GACCGCAGGCGCTCGCGGAAGCCTTGC-3′
PA2931_His_for	5′-GAAAATCTTCTCTCATCCGCCTCAATGATGATGATG ATGATGGGGCCAGGCGCGCAGCGCCCGTTCG-3′
PA2931_His_rev	5′-CTCCATACCCGTTTTTTTGGGCTAGCCCAAGGA AGCACACATATGGCAACGCGAGGCAGGCCAC-3′
EMSA_5′bt_for	5′-BT-CCTGACCTCCATTATTTGTATCGATCAC-3′
EMSA_5′bt_rev	5′-BT-GAGCAGGTTGGACATGGTCTTTTCC-3′
EMSA_for	5′-CCTGACCTCCATTATTTGTATCGATCAC-3′
EMSA_rev	5′-GAGCAGGTTGGACATGGTCTTTTCC-3′

^aBT, biotinylated.