TABLE 2.
Protocol 2 cytokine levels in the lungs of mice in response to the passive transfer of MAbs 30 days after i.t. infection with P. brasiliensis
| Cytokine | Days of infection | Cytokine level (pg/g of tissue)a
|
||||
|---|---|---|---|---|---|---|
| Sham | Infected only | Irrelevant MAb A4 | Treated with MAb 3E | Treated with MAb 32H | ||
| IL-4 | 45 | 208.2 ± 73.1 | 589.2 ± 81.9 | 342.3 ± 36.2 | 2,151.6 ± 352b | 1,370.6 ± 134b |
| 60 | 254.3 ± 85.7 | 862.8 ± 185.5 | 764.8 ± 107.8 | 1,633.2 ± 188b | 1,832.9 ± 287.6b | |
| IL-10 | 45 | 1,685.7 ± 252.5 | 2,541.9 ± 172.2 | 1,666.7 ± 250 | 3,245.4 ± 318.1b | 1,790.6 ± 240.6b |
| 60 | 2,360 ± 494.7 | 2,760.2 ± 612.3 | 3,238.1 ± 798.4 | 3,583.3 ± 279b | 2,500 ± 839.9 | |
| IL-12 | 45 | 3,257.6 ± 370.4 | 4,912.5 ± 817.4 | 8,362.5 ± 1,969.6 | 21,337.9 ± 3,475.9b | 12,470.1 ± 1,815.5b |
| 60 | 3,243.7 ± 1,885.6 | 6,987.4 ± 891.5 | 9,509.5 ± 3,249.4 | 14,699.3 ± 2,981.1b | 11,630.6 ± 2,350.8b | |
| IFN-γ | 45 | 99.41 ± 33.1 | 280.9 ± 41.2 | 106.6 ± 29.2 | 1,328.6 ± 207.2b | 162.5 ± 42.1b |
| 60 | 90.2 ± 25.4 | 382.4 ± 39.2 | 133.3 ± 33 | 1,229.8 ± 192.3b | 416.4 ± 59.3 | |
| TNF-α | 45 | 1,980.5 ± 359 | 22,870.5 ± 1,057.6 | 22,100 ± 3,225.5 | 16,985.4 ± 1,698.2b | 18,798.5 ± 2,785.2b |
| 60 | 2,010.5 ± 438.8 | 30,021 ± 3,777.8 | 26,789.2 ± 1,469.3 | 14,892.4 ± 2,001b | 21,364.7 ± 3,911.2b | |
a
Values are means ± standard deviations of measurements from 10 animals per group. The experiment was repeated twice with reproducible results.
b
Statistically significant (P < 0.05, determined by analysis of variance and Tukey's honestly significant difference test) relative to the value for the untreated, infected mice.