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. 1999 Dec 7;96(25):14511–14516. doi: 10.1073/pnas.96.25.14511

Figure 1.

Figure 1

GKRP gene. (a) Southern hybridization analysis of BamHI-digested DNA with promoter region probe identifying the normal (4.8-kb) and the mutant (2.3-kb) alleles. (b) Northern hybridization analysis of liver RNA showing the lack of compensation from the normal allele in +/− mice and the absence of a GKRP transcript in −/− mice. (c) Western blot analysis of liver extracts showing the ≈65-kDa GKRP in +/+ and +/− mice, decreased levels of GKRP in +/− mice, and the complete absence of GKRP in −/− mice. (d) Coomassie blue-stained SDS/PAGE gel showing equivalent amounts of protein loaded.