Figure 2.

GK gene expression in GKRP-mutant liver. (a) Glucose phosphorylation in liver extracts of fasted mice, showing reduced GK activity in GKRP mutants. mU, milliunits. (+/+, n = 8; +/−, n = 7; −/−, n = 7). *, P ≤ 0.05, +/+ versus +/− or −/−. (b) Representative Western blot analysis of liver extracts with GK antibodies showing the GK protein and decreased levels in +/− and in −/− livers. (c) Liver RNA analysis showing the absence of significant changes in GK mRNA levels in GKRP mutants. Data are presented as percentage of +/+ mice liver with +/+ taken as 100%. (+/+, n = 3; +/−, n = 3; −/−, n = 3). Mean ± SEM.