Figure 5.

Subcellular localization of GKRP and GK in mouse primary hepatocytes. Immunocytochemical analysis with anti-GKRP and anti-GK antibodies. (a–c) GKRP continued to remain in the nucleus of +/+ hepatocytes cultured in low-glucose- (a), low-fructose + low-glucose- (b), or in high-glucose-containing media (c). (d–f) GK is present in the nucleus of +/+ hepatocytes cultured in low-glucose-containing medium (d), translocated into the cytoplasm of +/+ hepatocytes cultured in low-fructose + low-glucose- (e) or in high-glucose-containing media (f). (g–i) GK present in the cytoplasm of −/− hepatocytes cultured in low-glucose medium (g) continued to stay in the cytoplasm in low-fructose + glucose- (h) or in high-glucose-containing medium (i).