TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| Francisella | ||
| Schu S4 | F. tularenis subsp. tularensis, wild-type | CDC |
| BJM1005 | Schu S4 EZ-Tn5<rpsL-arr-2>::dsbB | 33 |
| BJM1005C | BJM1005 containing pAQ012 | This study |
| BJM1032 | Schu S4 ΔacrB | This study |
| BJM1039 | Schu S4 ΔdsbB | This study |
| BJM1040 | Schu S4 ΔacrA | This study |
| BJM1041 | BJM1039 containing pAQ061 | This study |
| E. coli | ||
| JW5182-1 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) ΔdsbB774::Kan rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| BMAQ076 | JW5182-1 with pAQ061 | This study |
| BMAQ077 | JW5182-1 with pFNLTP-6-groE-gfp | This study |
| BW25113 | Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) rph-1 Δ(rhaD-rhaB)568 hsdR514 | 3 |
| BMAQ078 | BW25113 with pAQ061 | This study |
| BMAQ079 | BW25113 with pFNLTP-6-groE-gfp | This study |
| Plasmids | ||
| pGIR463 | sacB suicide vector | G. Ramakrishnan |
| pFNLTP-6-groE-gfp | 30 | |
| pAQ012 | dsbB pFNLTP-6-groE-gfp | |
| pDS033 | 5′- and 3′-flanking regions of acrB in pGIR463 | |
| pAQ052 | 5′- and 3′-flanking region of dsbB in pGIR463 | |
| pAQ061 | dsbB with 5′- and 3′-flanking regions in pFNLTP-6-groE-gfp | |
| pAQ072 | 5′- and 3′-flanking region of acrA in pGIR463 |