TABLE 2.

Primers used in this study^a

Primer	Sequence	Description	Restriction enzyme site
BM025	gcGAATTCaactatgatacaattac	F upstream of dsbB, for trans-complementation	EcoRI
BM026	aGAATTCaaggaggtacatatctaatgaa	F dsbB, for trans-complementation
BM028	cttaCTCGAGacagttttgtcc	R dsbB, for trans-complementation	XhoI
BM045	gatagcattcatacccttatgg	F ΔdsbB, to confirm deletion
BM047	ccagactgtgaagatatcg	RT-PCR
BM048	atctggcgttagtttaactg	RT-PCR
BM051	gtagacacatgggacatgg	RT-PCR
BM052	ctcagaaacacgctaaagc	RT-PCR, R to confirm ΔdsbB deletion
BM074	TCCGGAgactatctatctgcgctagc	F 5′-flanking ΔacrB	BspEI
BM075	GCGGCCGCatttctatattcttgagc	R 5′-flanking ΔacrB	NotI
BM076	GCGGCCGtgcatcaacaatttcagctg	F 3′-flanking ΔacrB	NotI
BM077	taCTCGAGactagaccaccgagaatccc	R 3′-flanking ΔacrB	XhoI
BM106	ttaaTCCGGAatatcattgaggcatcagccgc	F 5′-flanking ΔdsbB	BspEI
BM107	acaatacaGCGGCCGCttgaagtagacacatgg	R 5′-flanking ΔdsbB	NotI
BM108	acttttaGCGGCCGCtaccaaatcaatcaccgg	F 3′-flanking ΔdsbB	NotI
BM109	ggatCTCGAGttgtgactgtaattgtcgcgc	R 3′-flanking ΔdsbB	XhoI
BM125	aaaTCCGGAcgctatgtttactcaagatccc	F 5′-flanking ΔacrA	BspEI
BM126	tgtttaatGCGGCCGCaagtcttcttaacgtcc	R 5′-flanking ΔacrA	NotI
BM127	taattaaaGCGGCCGCataagggtatgaatgc	F 3′-flanking ΔacrA	NotI
BM128	ctgaCTCGAGcaacatcaacatttaagccattagg	R 3′-flanking ΔacrA	XhoI
BM129	gcaaatcaGCGGCCGCaacaatcaatagttgc	F ΔacrA, to confirm deletion	NotI
BM130	gatcCTCGAGacgcattactgaattccttgc	R ΔacrA, to confirm deletion	XhoI

^aUppercase type indicates restriction enzyme site. F, forward primer; R, reverse primer.