TABLE 3.
Sensitivities of mutants to drugs, detergents, and dyesa
| Drug, detergent, or dye | Dose (μg/disk) | Mean zone of inhibition (mm)
|
||||
|---|---|---|---|---|---|---|
| Schu S4b | BJM1039b (ΔdsbB) | BJM1032b (ΔacrB) | Schu S4c | BJM1040c (ΔacrA) | ||
| Ampicillin | 10 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| Carbenicillin | 10 | 6 ± 0 | 6 ± 0 | 6 ± 0 | ND | ND |
| Gentamicin | 5 | 25 ± 1 | 24 ± 0 | 26 ± 0 | 25.6 ± 0.58 | 27 ± 1 |
| Kanamycin | 5 | 20.7 ± 0.58 | 20.3 ± 0.58 | 19.7 ± 0.58 | 25.3 ± 0.58 | 27.6±.058 |
| Tetracycline | 10 | 22.3 ± 0.58 | 21.3 ± 1.2 | 27.3 ± 1.2 | 25.3 ± 1.2 | 27.3 ± 0.58 |
| Erythromycin | 50 | 12.7 ± 0.58 | 15 ± 0 | 29.3 ± 1.2 | 15.7 ± 0.58 | 33.3 ± 1.2 |
| Nalidixic acid | 10 | 30.7 ± 0.58 | 30 ± 0 | 32 ± 0 | 30.7 ± 1.2 | 33.3 ± 1.2 |
| Chloramphenicol | 50 | 40 ± 0 | 40 ± 0 | 40 ± 0 | 50.7 ± 1.2 | 50.7 ± 1.2 |
| Vancomycin | 25 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 | 6 ± 0 |
| Rifampin | 12.5 | 29.7 ± 0.58 | 30.3 ± 0.57 | 34 ± 0 | 30.7 ± 1.2 | 34.7 ± 1.2 |
| SDS | 56 | 10.7 ± 0.58 | 10.3 ± 0.57 | 23.7 ± 0.58 | 8.6 ± 0.58 | 22.3 ± 0.58 |
| Acriflavin | 10 | 26 ± 1 | 26.7 ± 0.57 | 30.7 ± 1.2 | 30 ± 2.0 | 33.3 ± 1.2 |
| Ethidium bromide | 25 | 25 ± 1 | 23.3 ± 0.57 | 34 ± 0 | 26.7 ± 1.2 | 34.7 ± 1.2 |
a
Sterile disks impregnated with the indicated amounts of compound were placed onto lawns of the indicated bacteria. Zones of growth inhibition were measured in millimeters at 48h. Values marked in boldface type indicate sensitivities that showed statistically significant increases (P ≤ 0.05) over levels of wild-type Schu S4. ND, not done; SDS, sodium dodecyl sulfate.
b
These experiments were performed together.
c
These experiments were performed together.