FIG. 4.
SdrG expression on S. epidermidis 0-47 in a murine model of bacteremia. A group of five mice was infected by intraperitoneal injection of 5 × 108 CFU 0-47. A 3 hours postinfection the blood from the mice was pooled and the bacteria isolated. The bacteria were then stained with rabbit anti-SdrGN23 immune serum and visualized with a confocal fluorescence microscope. In vitro-grown bacteria at the time of challenge were treated similarly and stained for comparison to the bacteria isolated from the bloodstreams of infected mice. Preincubation of the immune serum with rSdrG and staining with preimmune serum was included as a specificity control. Arrowheads point to the bacteria in the bright-field images. These data are representative of three independent experiments.