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Immunogold labeling of EF-Tu on intact M. pneumoniae cells. Mycoplasmas were incubated with antisera (1:1,000) generated against EN, ED3, or ED4 truncations followed by anti-mouse IgG gold complex (10 nm). Mycoplasma membrane-associated gold labeling of EF-Tu is readily observed with ED3 and ED4 antisera and much less so with EN antiserum. EN magnification, ×71,000; ED3 magnification, ×44,000; and ED4 magnification, ×71,000.
