TABLE 1.
Summary of rSj97 purification
| Purification step | Total protein (mg)a | Recovery (%)b | Activity (U)c |
|---|---|---|---|
| Inclusion body preparation | 6,570 | 100 | 0.0184 |
| Anion exchange | 1,234 | 18.8 | 0.209 |
| Hydroxyapatite | 506.3 | 7.71 | 0.367 |
| Size exclusion | 39 | 0.594 | 0.816 |
| Final processing | 22.4 | 0.341 | 1.00 |
a
Protein content was assessed by a BCA-based colorimetric assay.
b
Calculated as the percentage of the protein mass of intermediate purification steps relative to the yield from the inclusion body preparation.
c
Calculated from an ELISA-based collagen binding assay. Serial dilutions of rSj97 were utilized as a standard curve, with 1.0 U defined as the optical density corresponding to 12.5 μg/ml of purified rSj97.