FIG. 8.
Alpha-hemolytic activity and CNF1 expression in CP9 strains. Samples were prepared and assays were carried out as described in Material and Methods. (A) Alpha-hemolytic activity of CP9 or CP9ΔhlyA1::cat supernatants collected hourly from 0 to 48 h was measured by mixing the samples with sheep RBCs. Results for 9 to 17 and 25 to 48 h are not shown. C(−) and C(+) were the negative and positive controls, respectively. (B) Western blot analyses of CP9 and CP9ΔhlyA1::cat bacterial cell lysates probed with polyclonal anti-CNF1 serum.