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. 2008 Apr 21;76(7):2966–2977. doi: 10.1128/IAI.00323-08

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Copyright © 2008, American Society for Microbiology

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FIG. 1. — Preimmune chicken IgY recognized an unknown protein of ∼180 kDa in B. bronchiseptica. (A) WCLs of Bvg⁻-phase-locked strain RB54, Bvgⁱ-phase-locked strain RB53i, Bvg⁺-phase-locked strain RB53, and ΔfhaB strain RBX9 were probed with a chicken-derived polyclonal anti-FHA antibody (α-FHA). The arrowhead indicates FHA, and the asterisk denotes the 180-kDA protein. (B) WCLs of RB50 grown under Bvg⁺-phase conditions [RB50(+)]; RB50 grown under Bvg⁻-phase conditions [RB50(−)]; RB53, RB53i, RB54, and RBX9 grown under Bvg⁺-phase conditions [RBX9(+)]; and RBX9 grown under Bvg⁻-phase conditions [RBX9(−)] were probed with preimmune chicken IgY. (C) Outer membranes prepared from RB50 grown under Bvg⁺-phase conditions, RB50 grown under Bvg⁻-phase conditions, and RBX9 grown under Bvg⁺-phase conditions were probed with preimmune chicken IgY. (D) WCLs of RB50, RB50::pRH4, and RBAT grown under Bvg⁺-phase conditions were probed with preimmune chicken IgY.