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Relative batB mRNA levels in B. bronchiseptica. qRT-PCR was used to determine the relative levels of batB transcript in wild-type RB50 (light gray bar), Bvg+-phase-locked mutant RB53 (white bar), Bvgi-phase-locked mutant RB53i (dark gray bar), and ΔbvgS strain RB54 (black bar).