TABLE 1.
Strains and plasmids used in this analysis
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Used for molecular cloning | Gibco-BRL, Gaithersburg, MD |
| SM10λpir | Used for conjugation with Bordetella | 31 |
| B. bronchiseptica | ||
| RB50 | WT | 5 |
| RB53 | Bvg+-phase-locked derivative of RB50 | 5 |
| RB53i | Bvgi-phase-locked derivative of RB50 | 6 |
| RB54 | Bvg−-phase-locked derivative of RB50 | 5 |
| RBX9 | ΔfhaB derivative of RB50 | 7 |
| B. parapertussishu | ||
| 12822 | Clinical B. parapertussishu isolate | 20 |
| Plasmids | ||
| RB50::pRH4 | RB50 with plasmid pRH4 integrated, resulting in the disruption of ORF452 | This study |
| RB50::pCW85 | RB50 with B. bronchiseptica batB promoter region-lacZYA fusion | This study |
| RB50::pCW86 | RB50 with B. pertussis batB promoter region-lacZYA fusion | This study |
| RB50::pCW87 | RB50 with B. parapertussishubatB promoter region-lacZYA fusion | This study |
| RBAT | ΔbatB derivative of RB50 | This study |
| RBAT::pCW98 | Complemented ΔbatB strain | This study |
| RBatpp | RB50 with 1,593-bp deletion within batB | This study |
| BPSM | Streptomycin-resistant derivative of clinical B. pertussis isolate Tohama I | 30 |
| BPSM::pCW85 | BPSM with B. bronchiseptica batB promoter region-lacZYA fusion | This study |
| BPSM::pCW86 | BPSM with B. pertussis batB promoter region-lacZYA fusion | This study |
| BPSM::pCW87 | BPSM with B. parapertussishubatB promoter region-lacZYA fusion | This study |
| 12822::pCW85 | 12822 with B. bronchiseptica batB promoter region-lacZYA fusion | This study |
| 12822::pCW86 | 12822 with B. pertussis batB promoter region-lacZYA fusion | This study |
| 12822::pCW87 | 12822 with B. parapertussishubatB promoter region-lacZYA fusion | This study |