FIG. 3.
SHIP deficiency leads to altered levels of inflammatory cytokine production after Salmonella infection in vivo. ship+/+and ship−/− mice were infected orally with 106 serovar Typhimurium SL1344 (A), i.p. with 102 serovar Typhimurium SL1344 (B), or left uninfected (C) and sacrificed at 2 days postinfection or 5 days postinfection, respectively. Blood samples were obtained via cardiac puncture, and serum was separated for cytokine analysis. Cytokines were analyzed by using the flow cytometry-based CBA mouse inflammation kit. For panels A and B, three independent experiments were performed with a total of 12 animals for both ship+/+and ship−/− mice.