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. 2008 Apr 21;76(7):2913–2922. doi: 10.1128/IAI.01596-07

FIG. 4.

FIG. 4.

M2 macrophage markers are found in the guts and peritoneal cavities of ship−/− mice. (A and B) Sections of small intestine were taken from uninfected (ship+/+ and ship−/−) and orally infected ship+/+and ship−/− mice (ship+/+ SL1344 and ship−/− SL1344) at day 2 postinfection and stained for the M2 macrophage markers YM1 or Arg1 (green) or and F4/80 (red) as a macrophage control. (C) Sections of small intestine were taken from uninfected ship+/+ and ship−/− mice (−SL1344) and orally infected ship+/+and ship−/− mice (+SL1344) at day 2 postinfection and stained with hematoxylin and eosin to show the pathology. All photographs were taken at ×40 magnification. (D) Peritoneal macrophages were obtained from uninfected (−SL1344) or i.p.-infected (+SL1344) ship+/+and ship−/− mice at 2 days postinfection. Cells were washed, counted, and lysed directly into Western sample buffer for protein analysis. Both bands are specific for Arg1 (35). For panels A to D, the results of representative experiments are shown.