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. 2008 Apr 21;76(7):2913–2922. doi: 10.1128/IAI.01596-07

FIG. 7.

FIG. 7.

ship−/− BMDMs are not more susceptible to death in vitro upon infection with Salmonella. BMDMs were obtained from ship+/+and ship−/− mice and derived in the presence of FBS plus 2% mouse serum for 10 days. Cells were seeded and either left untreated (−), infected with serovar Typhimurium SL1344 (SL1344) or heat-killed serovar Typhimurium SL1344 (HK) at an MOI of 10, or treated with 100 ng of serovar Typhimurium LPS/ml (LPS) for 8 h (A) or 24 h (B). Cells were collected and stained with the cell death marker 7AAD and analyzed via flow cytometry. Three independent experiments were performed, with each treatment being performed in triplicate for a total nine wells for each treatment.