TABLE 1.
Oligonucleotides
| Namea | Target geneb | Sequence (5′-3′) |
|---|---|---|
| MelFF | melF | CAGAAGACGCGATCACGGCG |
| MelFR | GGGTCGGCGAACACTTCACC | |
| MelMF | melM | CCTGGACCTGCCGCGCCTTG |
| MelMR | GTTCAGCGCCGCCAGCGTGG | |
| MelNF | melN | CCCAGCGGCACATCACGGTG |
| MelNR | CGCTGTGGTGGGCGTAGCCG | |
| MelOF | melO | GACCCCTGCGCACCTCGCCG |
| MelOR | CCGTCTGACCATCCGCACCG | |
| MelPF | melP | CCCAACACTGTCCACCCTGG |
| MelPR | CGTTGGCGGTGCAGGTCATC | |
| MrlIF | mrlI | CCCGTTGGGACTGGCTAC |
| MrlIR | GCCGAGGAGGTGCTATGA | |
| SigAF | sigA | CCTCGACCTTGACGCCGCCG |
| SigAR | GGCGCAGGTTGGCTTCCAGC | |
| rRNAF | 16S rRNA | AGAGTTTGATCCTGGCTCAG |
| rRNAR | CACGCTCACAGTTAAGCTGT | |
| MelDF | melD | CTAGCGCAGCCTGGTAGTC |
| MelDR | GCAAGAACCTGCATCTGGCC | |
| MelF2F | melF | TCGGAATATTCCTCATGCCG |
| MelF2R | GTTGGTCTTCGGTCCAGATC | |
| MelLF | melL | TATAGCTAGCGAACCAAGCAGCAATGACAG |
| MelLR | TATATTAATTAAACAACATCAGGCCCAGAAAG | |
| MrlACF | mrlA-C | TATATTAATTAACGGCTGACTTCCCGGATTCC |
| MrlACR | TATATTAATTAACGAGGCCATGGTCGACCAGG | |
| MrlFOF | mrlF-O | TATATTAATTAAAGCCACCGGACATACGAC |
| MrlFOR | TATAAGTACTGTGCCGTTCCCACAGTCC | |
| MrlJOF | mrlJ-O | TATATTAATTAAACCGTCGAGGTGATCGTG |
| MrlJOR | TATAAGTACTGTGCCGTTCCCACAGTCC |
a
Suffixes: F, forward primer; R, reverse primer.
b
That is, the target gene mRNA transcript for RT-PCR analyses or PCR or target genes for the construction of the complementation constructs pJDC117 (melL), pJDC119 (mrlA-C), pJDC120 (mrlF-O), and pJDC121 (mrlJ-O). Oligonucleotides are listed as primer pairs. Reverse primers were used for cDNA synthesis, and both forward and reverse primers were used for RT-PCR and PCR cloning.