TABLE 3.
Infection of murine macrophages
| Strain | Mean ± SD
|
|||
|---|---|---|---|---|
| % Infected cellsa | No. of vacuoles/cellb | No. of bacteria/vacuolec | Infectivity indexd | |
| M. marinum | 14 ± 1.0 | 1.2 ± 0.6 | 10 ± 7.8 | 1.0 ± 0.10 |
| M. marinum::1-B12 | 51 ± 4.7 | 2.1 ± 1.3 | 15 ± 9.8 | 9.6 ± 0.77* |
| M. marinum (melL) | 3.7 ± 0.9 | 1.1 ± 0.2 | 9.5 ± 6.8 | 0.2 ± 0.05* |
| M. marinum::2-G2 | 49 ± 5.2 | 2.1 ± 1.6 | 12 ± 8.3 | 7.4 ± 0.79* |
| M. marinum::4-B2 | 6.1 ± 2.3 | 1.0 ± 0.4 | 16 ± 13 | 0.6 ± 0.23† |
| M. marinum (mrlA) | 30 ± 5.3 | 2.2 ± 1.3 | 11 ± 6.0 | 4.3 ± 0.76* |
| M. marinum::4-H9 | 7.2 ± 1.1 | 1.1 ± 0.3 | 13 ± 9.1 | 0.6 ± 0.09* |
| M. marinum (mrlG) | 37 ± 2.1 | 1.9 ± 1.4 | 11 ± 8.3 | 4.6 ± 0.06* |
| M. marinum (mrlL) | 28 ± 2.5 | 1.1 ± 0.6 | 14 ± 12 | 2.6 ± 0.23* |
That is, the percentage of cells containing at least one bacterium in a field. The results are the means ± standard deviations for three counts of 25 fields.
That is, the number of bacterial vacuoles in each cell. Results are the means for three counts of 50 cells.
That is, the number of bacteria in each vacuole. Results are means ± the standard deviations for three counts of 50 cells.
The infectivity index was calculated as follows: (% infected cells × the number of vacuoles per cell × the number of bacteria per vacuole for the strain)/(% infected cells × the number of vacuoles per cell × the number of bacteria per vacuole for M. marinum). *, Significantly different from wild type M. marinum (P < 0.01); †, significantly different from wild-type M. marinum (P < 0.05).