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TOP52 ΔfimK and TOP52/pfimX appear hyperpiliated by negative-stain EM. Negative-stain EM was performed on TOP52 cultures, and representative images are displayed. TOP52 had low to moderate piliation, and the negative control TOP52 ΔfimA-fimH appeared bald. TOP52 ΔfimK was hyperpiliated compared to the wild type, and this phenotype could be partially complemented with pfimK. TOP52/pfimX also displayed higher levels of piliation than the vector control.
