FIG. 6.

The presence of fimK and fimX affects biofilm formation of TOP52 and UTI89. A 48-h biofilm assay was used to quantify biofilms produced by TOP52 strains (A) and UTI89 strains (B). TOP52 and the negative control TOP52 ΔfimA-fimH failed to form biofilms. However, TOP52 ΔfimK and TOP52/pfimX were able to form biofilms. TOP52 ΔfimK/pfimK formed significantly less biofilm than the vector control. Wild-type UTI89 formed a robust biofilm, and the negative control UTI89 ΔfimA-fimH was unable to form a biofilm. Complementation of UTI89 with TOP52 fimK resulted in significantly less biofilm formation than that produced by the UTI89/pBAD vector control. Data combine means of two independent experiments, each with duplicate plates. Error bars represent standard errors of the means.