TABLE 2.
Primer sequences
| Primer name | Primer use | Primer sequence |
|---|---|---|
| NotIFimKF1 | Amplify 1-kb fimK 5′ flanking region | 5′-CGGTAACGCGGCCGCGACTATCCGGAAACGATCACC-3′ |
| HindIIIFimKR2 | Amplify 1-kb fimK 5′ flanking region | 5′-AACAAGCTTAGACGATCCGGATGACTCAC-3′ |
| HindIIIFimKF3 | Amplify 1-kb fimK 3′ flanking region | 5′-CATAAGCTTAGAAAAGCGCACCGGTTA-3′ |
| SalIFimKR4 | Amplify 1-kb fimK 3′ flanking region | 5′-AACGTCGACAAAACAGAAACCACAGCAACG-3′ |
| 3FimKCheckF | Check TOP52 ΔfimK | 5′-GTCGATTATCGGCATCACCT-3′ |
| 3FimKCheckR | Check TOP52 ΔfimK | 5′-GTGGCGAAGGTAGTGGAAAA-3′ |
| NotIFimF1 | Amplify 1-kb fimA 5′ flanking region | 5′-CGGTAACGCGGCCGCGCATTAGCGAACTGCTGGAT-3′ |
| BamHIFimR2 | Amplify 1-kb fimA 5′ flanking region | 5′-AACGGATCCCAGGGCTGACACAACAATCA-3′ |
| BamHIFimF3 | Amplify 1-kb fimH 3′ flanking region | 5′-TACGGATCCGTCAATCTCGGCCTGACG-3′ |
| SalIFimR4 | Amplify 1-kb fimH 3′ flanking region | 5′-AACGTCGACGGAGGGTATGTTGACCGAAA-3′ |
| 3FimCheckF | Check TOP52 ΔfimA-fimH | 5′-CCAACGCCATCGCTATTC-3′ |
| 3FimCheckR | Check TOP52 ΔfimA-fimH | 5′-GATACACCACGATCCGCTTC-3′ |
| 3FimKcompF | Amplify TOP52 fimK for cloning into pBAD | 5′-AAT TCC ATG GGGCATCACCTTTGTCTATCAATGATG-3′ |
| 3FimKcompR | Amplify TOP52 fimK for cloning into pBAD | 5′-AATTGGTACCCGGTGCGCTTTTCTCGCACCCTCAACG-3′ |
| pBADF | Check pBAD clones | 5′-TATCGCAACTCTCTACTGTTTCTCCA-3′ |
| pBADR | Check pBAD clones | 5′-CTGTATCAGGCTGAAAATCTTCTCTCA-3′ |
| UTI89fimA-H KO F | Knockout fimA-fimH in UTI89 | 5′-ATGAAAATTAAAACTCTGGCAATTGTTGTTCTGTCGGCTCTGTCCCTGAGCATATGAATATCCTCCTTAG-3′ |
| UTI89fimA-H KO R | Knockout fimA-fimH in UTI89 | 5′-TTATTGATAAACAAAAGTCACGCCAATAATCGATTG CACATTCCCTGCAGGTGTAGGCTGGAGCTGCTTC-3′ |
| UTI89fimA-HcheckF | Check UTI89 ΔfimA-fimH | 5′-GTTTGTGCGCGATGCTTTCC-3′ |
| UTI89fimA-HcheckR | Check UTI89 ΔfimA-fimH | 5′-GAGCACCTGAGCCTGCCATT-3′ |
| 2KlebphaseF | Amplify fimS region for phase assay | 5′-GGGACAGATACGCGTTTGAT-3′ |
| 2KlebphaseR | Amplify fimS region for phase assay | 5′-GGGACAGATACGCGTTTGAT-3′ |