TABLE 3.
Serological titers required to agglutinate K. pneumoniae strains using monospecific antiserum to type 1 pili
Strain | Growth with arabinose | Titera |
---|---|---|
TOP52 | − | 160 |
TOP52 ΔfimK | − | 2,560 |
TOP52 ΔfimK/pBAD | − | 1,280 |
TOP52 ΔfimK/pBAD | + | 2,560 |
TOP52 ΔfimK/pfimK | − | 1,280 |
TOP52 ΔfimK/pfimK | + | 80 |
TOP52 ΔfimA-fimH | − | <20b |
Titers are the reciprocal of the highest dilution of serum causing visible agglutination after 5 min. Titers represent the median of three independent experiments.
The lowest dilution of antibody used in this assay was 1:20.