FIG. 5.
IFN-γ-producing CD8+ T cells in spleens of mice after infection with B. abortus 2308 or the virB mutant. C57BL/6 mice (four mice per group) were injected i.p. with PBS or 5 × 105 CFU of B. abortus 2308 or the virB mutant and sacrificed 3 days after infection. Splenocytes were stained as described in the legend of Fig. 4. One million cells/sample were acquired with an LSRII apparatus. Numbers indicate the percentages of cells in each gate. Data for one mouse per group are shown as a representative of the group. SSC, side scatter.