FIG. 6.

Percentage of splenic CD4⁺ T cells or CD8⁺ T cells secreting IFN-γ after infection with B. abortus 2308 or the virB mutant. C57BL/6 mice (four mice per group) were injected i.p. with PBS or 5 × 10⁵ CFU of B. abortus 2308 or the virB mutant. Mice were sacrificed 3 days after infection, and cells/mouse were stained for IFN-γ-producing cells. One million cells/sample were acquired with an LSRII apparatus. The asterisk represents statistical significance determined using a Student's t test on data after logarithmic conversion.