TABLE 2.
Plasmids used in this study
| Plasmid | Relevant characteristic(s) | Derivation or reference |
|---|---|---|
| pIN-III-ompA | Secretion vector with ompA signal sequence | 50 |
| TOPO-TA | Commercial vector for TA cloning | Invitrogen |
| pYA3342 | Asd+ expression vector Ptrc promoter pBRori | 39 |
| pYA3493 | Asd+ vector bla SS-based secretion periplasmic plasmid pBRori | 39 |
| pYA3620 | Asd+ vector bla SS+CT-based secretion periplasmic plasmid pBRori | 14 |
| pYA3744 | 1.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA4106 | This study |
| pYA3802 | 0.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA3620 | This study |
| pYA4028 | 1.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA3493 | This study |
| pYA4088 | 0.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA3493 | This study |
| pYA4096 | His-tagged PspC (aa 4-404) in pET28a | This study |
| pYA4098 | 1.5-kb DNA encoding the codon-optimized α-helical and proline-rich regions of PspC (aa 4-477) in TOPO vector | This study |
| pYA4102 | Asd+ vector ompA SS-based plasmid pBRori | This study |
| pYA4106 | Asd+ vector phoA SS-based plasmid pBRori | This study |
| pYA4202 | 1.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA4102 | This study |
| pYA4266 | 0.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA4102 | This study |
| pYA4267 | 0.8-kb DNA encoding the α-helical region of PspA (aa 3-285) in pYA4106 | This study |
| pYA4269 | 1.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA3620 | This study |
| pYA4270 | 1.2-kb DNA encoding the α-helical region of PspC (aa 4-404) in pYA3342 | This study |
| UAB055 | α-helical region of PspA (aa 1-302) in pET20b | 3 |