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. 2008 May 5;76(7):3093–3099. doi: 10.1128/IAI.01627-07

FIG. 3.

FIG. 3.

Regulation of gbpC expression by SMu486/SMu487. (A) Expression from PgbpC-gusA was measured for the wild type, (IBS131); the ΔSMu486 (IBS151), ΔSMu487 (IBS152), and ΔSMu486/SMu487 (IBS155) strains; the ΔSMu486 strain complemented with pIB55 (IBS151/pIB55); and the single-crossover ΔSMu486 strain (IBS339). Experiments were performed in triplicate, and the mean values with standard deviations are shown. (B) Expression from PgbpC-gusA was measured with the wild-type strain (IBS131) containing either pIB169 or pIB181, as described in the text. Experiments were performed in triplicate, and the mean values with standard deviations are shown. (C) Semiquantitative RT-PCR results showing the expression of gbpC from the wild type (UA159), the ΔSMu486 mutant (IBS148), the ΔSMu487 mutant (IBS149), and the ΔSMu486 mutant complemented with pIB55 (IBS148/pIB55). The data are representative of RT-PCR analyses resulting from at least two different RNA isolations.