Cytokine profiles from P. gingivalis challenged or nasally immunized serum samples collected after 11 (A) and 14 (B) weeks. The level of each cytokine is presented as the percentage of the positive control. The data represent the mean ± the SD of three independent experiments of three pooled samples. ★, P < 0.01; ★★, P < 0.05 (compared to the control group). #, P < 0.01; ##, P < 0.05 (compared to the P. gingivalis-challenged group). Abbreviations: GCSF, granulocyte colony-stimulating factor; GMCSF, granulocyte-macrophage colony-stimulating factor; IFN-γ, gamma interferon; TNF-α, tumor necrosis factor alpha; VEGF, vascular endothelial growth factor.