TABLE 2.
Primers used in this study
| Locusa or primer name | Sequence (5′-3′) of indicated primera:
|
Use | |
|---|---|---|---|
| Forward | Reverse | ||
| EF_0082 | TTGACGTCAGCACCTTCTTC | CGTAGCGTTCACCTTTGACA | RT-qPCR |
| arcA | CGGTGAACACCGTAAATTCAT | AAACAACCAAACCACCTTCG | RT-qPCR |
| arcB | ACTGTTTGCCAGCCTTTCAT | GCGGAAGACTTCATCCGTAA | RT-qPCR |
| arcC-1 | TTGTCCAATGCGCTTAATCA | ATGCCTCATCTGCTGGATCT | RT-qPCR |
| EF_1459 | TTTGCTAACAGTCAATTGGATTACA | TGCTTTATTATTTTGCAGGGTTT | RT-qPCR |
| citF | ACTTGTTCGTGGACGGATTC | TGCAATGCCGACTTCTGTTA | RT-qPCR |
| Prom-ers | TAAGTTTGGTTCTGTCATTA | CGACTCGAAAGGAATGTTCA | Cloning in pGEM-T |
| Prom-ef0082 | CTTTTTCTATGTATGAGGAAG | CACCGCAATAATAACCATTA | Cloning in pGEM-T |
| Prom-arcAb | CTGTCGCTGGCTTCGACACTGTTA | CACTGTTTTCAATTTCCGATTTCAG | Cloning in pGEM-T |
| Prom-ef1459b | CAGTGATGACCTAGTGAGGTTTGC | CTGTGTAATCCAATTGACTGTTAGC | Cloning in pGEM-T |
| Prom-citfb | ATTGCGTTAGCATTGGCGGC | CTTCCTAAAAGTTTTGTCTCCCGAG | Cloning in pGEM-T |
| Prom-ef3326b | GTAAGCGTTAACAACCGGTGTTAA | CCTGTGTGTTGCTCTTTCTTG | Cloning in pGEM-T |
| ersDC-Uc | CCGGAATTCCCATGATCTAACTGTAACGGTGTCC | CGCGGATCCTAGTCTTGTAAAACATTTCTCATTGGC | Cloning in pMAD |
| ersDC-Dc | CGCGGATCCTTAACATCAGCACCCCCCGCAAGT | GAAGATCTGGGGGATATTATGGATGTGCTAGG | Cloning in pMAD |
| arcA-intd | TTGACGACAACGAAGAACTGATTCAA | CGGTGTTCACCGATATCAAAAGCC | Cloning in pORI19-1 |
| citF-intd | TCTTGGATATGCGATGGTCGATG | GCGGTGAGGCATACATATTGGC | Cloning in pORI19-1 |
| ef1459-intd | AAAGCATGTAGTTGATTTTGCTAAC | TTCTCCACTTTTTATAAGCATCG | Cloning in pORI19-1 |
| pQE30-Ers | GCGGATCCATGAGAAATGTTTTACAAGACTAC | CGCGCGCTGCAGTTAAACAATAATGTTATCTCTAATC | Cloning in pQE30 |
| mad1F | TCTAGCTAATGTTACGTTACAC | Cloning verification | |
| mad2R | TCATAATGGGGAAGGCCATC | Cloning verification | |
| Pu | GTTGTAAAACGACGGCCAGT | Cloning verification | |
| Pr | CACAGGAAACAGATATGACC | Cloning verification | |
a
From the annotated sequence available at http://www.tigr.org.
b
Primers also used for RT-PCR experiments.
c
Primers used to clone upstream (U) and downstream (D) sequences of ers in order to construct a deleted mutant by a double crossing-over (DC) event.
d
Primers used to clone internal fragments of arcA, citF, and EF_1459 in order to construct insertional mutants.