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. 2008 Apr 21;76(7):2852–2861. doi: 10.1128/IAI.01726-07

FIG. 2.

FIG. 2.

LVS pilF and pilT mutants. (A) Construction and validation of the DTLB (ΔpilF) mutant. PCR was performed with primers internal or external to the pilF gene. Lanes: 1, intermediate strain; 2, final deletion strain DTLB; 3, allelic replacement plasmid pPVLB; 4, LVS. (B and C) Schematic representations of the pilF and pilT transposon insertion mutations. (B) The HimarFT transposon inserted at bp 1169 of pilF. (C) The HimarFT transposon inserted at bp 127 of the first pilT ORF (FTL_1771). The aphA-2 gene for kanamycin resistance in the transposon is under control of the groE promoter, which could drive expression of downstream genes.