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. 2008 Apr 21;76(7):2852–2861. doi: 10.1128/IAI.01726-07

FIG. 3.

FIG. 3.

Whole bacteria, negative-stain TEM of F. tularensis LVS. (A and B) The wild-type LVS expresses pilus fibers. (C and D) No fibers were detected on strain DTLB (ΔpilF). (E and F) Complementation of strain DTLB with plasmid pGPB (pilF+) restored the expression of pili. (G and H) The pilT::HimarFT mutant expresses no pilus fibers. (I through L) Complementation of the LVS pilT::HimarFT mutant with pilT from either the LVS (p633TL; panels I and J) or F. tularensis subsp. novicida (p633TN; panels K and L) restored the expression of pili.