TABLE 2.
Primers used in this study
| Primer | Sequence (5′-3′)a | Features |
|---|---|---|
| UF3254F | ACA AGT CGA CAT CAA TAT AAC TAA AGT CTC TAG GC | Primer for cloning upstream pilF with the SalI site |
| UF4240R | ATG ACT GCA GCT TTT GCG TGT AAA TAG TGC | Primer for cloning upstream pilF with the PstI site |
| DF5633F | ATT GCT GCA GAA GCT CAA AAA GAA GGA ATC G | Primer for cloning downstream pilF with the PstI site |
| DF6650R | ACT TGT CGA CTC CCG TTG CTA AAG ACA CC | Primer for cloning downstream pilF with the SalI site |
| FG4116F | GCA AAT GTT GGC ATA TTA CC | Downstream pilF primer (inside pilC) |
| FG6231F | AAA TCG CTG TTG GCA CCT C | Upstream pilF primer |
| FG4501R | ACT TTA TCT AGA GTT GTT CC | pilF internal primer |
| FG4609F | CTA ACT CGA AAG CTA TGT CC | pilF internal primer |
| SP6 | CGA TTT AGG TGA CAC TAT AG | Sequencing insert in pGEM-Easy vector |
| T7 | TAA TAC GAC TCA CTA TAG GG | Sequencing insert in pGEM-Easy vector |
| SAC1243R | TTC CTT TCG CTT GAG GTA CAG C | Detecting sacB (S1) |
| SAC787F | GCA AAC ACT GGA ACT GAA GAT GG | Detecting sacB (S2) |
| PILT186F | GCT GAT AGA AAC CTA CGA ATG TG | Detecting pilT (T1) |
| PILT682R | TCC CTA AAA CCA AAT GCC C | Detecting pilT (T2) |
| PilB F3845 | CAA AGC TAG CAG CTA TAG CCT CCT CCT AAG CTA | Primer for cloning pilF with NheI site |
| PilB R5718 | CCC TGG ATC CTT AAC TTA CAC GGT ATA CTT C | Primer for cloning pilF with BamHI site |
| PilTFCOM | GAA AGA TAT CCT ATT TAA GTA ATC AAT CTG A | Primer for cloning pilT with EcoRV site |
| PilTRCOM | AGG AAC GCG TTT ATC TTA CTA CAA TGT ATT TAG CGG C | Primer for cloning pilT with MluI site |
a
Boldface letters indicate restriction sites.