FIG. 2.
Cloning and organization of the PHA synthase genes in H. mediterranei. (A) Cloning of the PHA synthase genes by TAIL-PCR. The left and right gels represent the second- and third-round (II and III) PCR products, upstream and downstream of the known partial phaC sequence, respectively. The interlaced specific primers (dp1 to -3 and up1 to -3) (Table 2) were designed based on the obtained partial phaC sequence (black box). AD, arbitrary degenerate primers (AD1 to -3) (Table 2). (B) Gene organization of the cloned DNA region. ORF1 encodes an unknown protein; ORF2 and ORF3 encode PhaEHme and PhaCHme, respectively; ORF4 encodes a universal stress protein (COG0589).