TABLE 2.
Oligonucleotides used in this study as primers for PCR
| Primer | 5′-3′ sequencea |
|---|---|
| P1 | CTCATCGTTTACGCGCTCATCAAC |
| P2 | GGCCTCCGGCGGGATGAGGTGGTC |
| AD1 | NGTCGASWGANAWGAA |
| AD2 | AGWGNAGWANCAWAGG |
| AD3 | NTCGASTWTSGWGTT |
| dp1 | TACGTCCGGTTGTTCGAG |
| dp2 | GCAGTTCTTGGAGGACGT |
| dp3 | CCAGATTGTCGGGGAGTA |
| up1 | CCATGCAGTACCCGAGAA |
| up2 | CGTGAGGTGGGTGTCGAG |
| up3 | GGTTGGAGGTCGAGAATG |
| KF1 | AGCCTGCAGCGGGTACGGGTGTTTCAG |
| KR1 | ATAGGATCCACAGACTACTCCGGCGTG |
| KF2 | ATAGGATCCGGCGAGTAAACCGTTCAA |
| KR2 | ATAGGTACCATCGGGAGCTGTACGGAC |
| phaEF1 | AGACGCCATGGACGAAAT |
| phaER1 | ATAGGATCCTCATCCCTCCACGTCCAT |
| phaCR1 | ATAGGATCCATCGGGAGCTGTACGGAC |
| phaCF1 | ATAGGATCCATGACACCAGTAACCTTCGC |
| phaCR2 | TCGGAGTTCCAGGTACCA |
| PER | ATAGGATCCGGGCATATACTCTCGGGC |
| phaCRS1 | ATAGGATCCTTATGAACGTTCGGCGAACCA |
| phaEFH | CCGACATATGTCACAACAAAAAGGGGA |
| phaERH | TTAGGTACCTTAGTGGTGGTGGTGGTGGTGTCCCTCCACGTCCATCGC |
| phaCFH | CCGACATATGACACCAGTAACCTTCGC |
| phaCRH | TTAGGTACCTTAGTGGTGGTGGTGGTGGTGGTCGGTGCGGTCGGATGC |
| PL | CGGGTACGGGTGTTTCAG |
| PR | ATCGGGAGCTGTACGGAC |
a
Sequences representing restriction sites are underlined.