TABLE 3.
PHA accumulation in H. mediterranei and H. hispanica strainsa
| Strain | Medium | Cell dry wt (g/liter) | PHBV content (% [wt/wt]) | 3HV fraction (mol%) | PHBV concn (g/liter) |
|---|---|---|---|---|---|
| H. mediterranei | AS-168 | 7.33 ± 0.51 | 18.21 ± 1.88 | 9.33 ± 0.13 | 1.33 ± 0.05 |
| H. mediterranei | MST | 7.01 ± 0.35 | 24.88 ± 1.27 | 13.37 ± 0.73 | 1.74 ± 0.04 |
| ΔphaECHme(pWL3E) | MST | 9.03 ± 0.14 | ND | ND | ND |
| ΔphaECHme(pWL3C) | MST | 9.27 ± 0.15 | ND | ND | ND |
| ΔphaECHme(pWL3EC) | MST | 9.02 ± 0.15 | 14.94 ± 0.24 | 11.85 ± 0.16 | 1.35 ± 0.04 |
| ΔphaECHme(pWLEC) | MST | 8.43 ± 0.31 | 17.26 ± 0.08 | 11.78 ± 0.44 | 1.38 ± 0.17 |
| H. hispanica | MG | 4.70 ± 0.23 | 12.26 ± 0.52 | 3.18 ± 0.35 | 0.58 ± 0.03 |
| PHB-1(pWL3E) | MG | 4.14 ± 0.10 | ND | ND | ND |
| PHB-1(pWL3C) | MG | 4.20 ± 0.21 | ND | ND | ND |
| PHB-1(pWL3EC) | MG | 5.27 ± 0.61 | 17.33 ± 0.04 | 3.14 ± 0.13 | 0.99 ± 0.11 |
| PHB-1(pWL3ECS-1) | MG | 6.39 ± 0.43 | 0.41 ± 0.02 | ND | 0.03 ± 0.00 |
a
The cells were cultured at 37°C for 72 h. The data are shown as mean ± standard deviation; n = 3. ND, not detectable.